rabbit anti myd88 pab  (Bioss)

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Xiaoyaosan Exerts Therapeutic Effects on the Colon of Chronic Restraint Stress Model Rats via the Regulation of Immunoinflammatory Activation Induced by the TLR4/NLRP3 Inflammasome Signaling Pathway

doi: 10.1155/2021/6673538

Figure Lengend Snippet: Primer sequences used for RT-qPCR.

Article Snippet: The membranes were then blocked with 5% skimmed milk powder at room temperature for 1 h and incubated with the following antibodies: anti-TLR4 rabbit pAb (Zen, 1 : 1,000); anti-TRAF6 rabbit pAb (Zen, 1 : 1,000); anti-TAK1 rabbit pAb (Zen, 1 : 1,000); anti-NF- κ B-p65 mAb (Zen, 1 : 1,000); anti-MYD88 rabbit pAb (Zen, 1 : 800); anti-IRAK1 (PTG, 1 : 1,000); anti-ASC (Bioss, 1 : 1,000); anti-NLRP3 (Bioss, 1 : 1,500); and anti-caspase-1 (Bioss, 1 : 800).

Techniques:

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Xiaoyaosan Exerts Therapeutic Effects on the Colon of Chronic Restraint Stress Model Rats via the Regulation of Immunoinflammatory Activation Induced by the TLR4/NLRP3 Inflammasome Signaling Pathway

doi: 10.1155/2021/6673538

Figure Lengend Snippet: The effect of Xiaoyaosan on the immunohistochemistry of TLR4, MyD88, NF- κ B-p65, TAK1, IRAK1, and TRAF6 in the colon. (a)–(f) Immunohistochemical analysis of TLR4, MyD88, NF- κ B-p65, TAK1, IRAK1, and TRAF6 expression in colonic tissue (×200 and ×400 magnification) and quantification of TLR4, MyD88, NF- κ B-p65, TAK1, IRAK1, and TRAF6 immunohistochemical staining. Bars represent the means ± SD of 5 rats per group. ▲ p < 0.05 and ▲▲ p < 0.01 vs. the control group; ∗ p < 0.05 and ∗∗ p < 0.01 vs. the model group. TLR4, Toll-like receptor 4; MyD88, myeloid differentiation primary response protein; IRAK1, interleukin 1 receptor-associated kinase1; TAK1, transforming growth factor beta-activated kinase 1; TRAF6, tumor necrosis factor receptor-associated factor 6; NF- κ B-p65, nuclear factor kappa beta-p65.

Article Snippet: The membranes were then blocked with 5% skimmed milk powder at room temperature for 1 h and incubated with the following antibodies: anti-TLR4 rabbit pAb (Zen, 1 : 1,000); anti-TRAF6 rabbit pAb (Zen, 1 : 1,000); anti-TAK1 rabbit pAb (Zen, 1 : 1,000); anti-NF- κ B-p65 mAb (Zen, 1 : 1,000); anti-MYD88 rabbit pAb (Zen, 1 : 800); anti-IRAK1 (PTG, 1 : 1,000); anti-ASC (Bioss, 1 : 1,000); anti-NLRP3 (Bioss, 1 : 1,500); and anti-caspase-1 (Bioss, 1 : 800).

Techniques: Immunohistochemistry, Immunohistochemical staining, Expressing, Staining

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Xiaoyaosan Exerts Therapeutic Effects on the Colon of Chronic Restraint Stress Model Rats via the Regulation of Immunoinflammatory Activation Induced by the TLR4/NLRP3 Inflammasome Signaling Pathway

doi: 10.1155/2021/6673538

Figure Lengend Snippet: The effects of XYS on the mRNA and protein expression of TLR4, MyD88, NF- κ B-p65, TAK1, IRAK1, TRAF6, NLRP3, caspase-1, and ASC in the rat colon. (a) The effects of XYS on the mRNA expression levels of Tlr4, Myd88, NF-κB-p65, Tak1, Irak1, Traf6, Nlrp3, caspase-1, and Asc in the rat colon. For Tlr4, Myd88, NF-κB-p65, Tak1, Irak1, and Traf6, data are expressed as the means ± SD of 5 rats per group. ∗ p < 0.05 and ∗∗ p < 0.01 vs. the model group; ▲ p < 0.05 and ▲▲ p < 0.01 vs. the control group. For Nlrp3 , caspase-1, and Asc , data are expressed as the means ± SD of 3 rats per group. ∗ p < 0.05 and ∗∗ p < 0.01 vs. the model group; ▲ p < 0.05 and ▲▲ p < 0.01 vs. the control group. (b)-(c) Semiquantitative Western blot analysis of TLR4, MyD88, NF- κ B-p65, TAK1, IRAK1, and TRAF6 expression in colonic tissue; data are expressed as the means ± SD of 5 rats per group. ▲ p < 0.05 and ▲▲ p < 0.01 vs. the control group; ∗ p < 0.05 and ∗∗ p < 0.01 vs. the model group. Semiquantitative Western blot analysis of NLRP3, ASC, and caspase-1 expression in colonic tissue; data are expressed as the means ± SD of 3 rats per group. ▲ p < 0.05 and ▲▲ p < 0.01 vs. the control group; ∗ p < 0.05 and ∗∗ p < 0.01 vs. the model group. TLR4, Toll-like receptor 4; MyD88, myeloid differentiation primary response protein; IRAK1, interleukin 1 receptor-associated kinase 1; TAK1, transforming growth factor beta-activated kinase 1; TRAF6, tumor necrosis factor receptor-associated factor 6; NF- κ B-p65, nuclear factor kappa beta-p65; NLRP3, NOD-like receptor protein 3; ASC, apoptosis-associated speck-like protein; XYS, Xiaoyaosan; FLU, fluoxetine.

Article Snippet: The membranes were then blocked with 5% skimmed milk powder at room temperature for 1 h and incubated with the following antibodies: anti-TLR4 rabbit pAb (Zen, 1 : 1,000); anti-TRAF6 rabbit pAb (Zen, 1 : 1,000); anti-TAK1 rabbit pAb (Zen, 1 : 1,000); anti-NF- κ B-p65 mAb (Zen, 1 : 1,000); anti-MYD88 rabbit pAb (Zen, 1 : 800); anti-IRAK1 (PTG, 1 : 1,000); anti-ASC (Bioss, 1 : 1,000); anti-NLRP3 (Bioss, 1 : 1,500); and anti-caspase-1 (Bioss, 1 : 800).

Techniques: Expressing, Western Blot

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Xiaoyaosan Exerts Therapeutic Effects on the Colon of Chronic Restraint Stress Model Rats via the Regulation of Immunoinflammatory Activation Induced by the TLR4/NLRP3 Inflammasome Signaling Pathway

doi: 10.1155/2021/6673538

Figure Lengend Snippet: Xiaoyaosan exerts its antidepressive effects in rats with chronic restraint-induced stress via regulating the activation of the immunoinflammatory response induced by the TLR4/NLRP3 inflammasome signaling pathway in the colon. Xiaoyaosan treatment reduced the expression levels of TLR4, MyD88, NF- κ B-p65, TAK1, IRAK1, TRAF6, NLRP3, ASC, and caspase-1 in colonic tissue of depressed rats; reduced the serum levels of the inflammatory factors IL-6, IL-1 β , and TNF- α ; and ameliorated colonic inflammation. TLR4, Toll-like receptor 4; MyD88, myeloid differentiation primary response protein; IRAK1, interleukin 1 receptor-associated kinase 1; TAK1, transforming growth factor beta-activated kinase 1; TRAF6, tumor necrosis factor receptor-associated factor 6; NF- κ B-p65, nuclear factor kappa beta-p65. NLRP3, NOD-like receptor protein 3; ASC, apoptosis-associated speck-like protein.

Article Snippet: The membranes were then blocked with 5% skimmed milk powder at room temperature for 1 h and incubated with the following antibodies: anti-TLR4 rabbit pAb (Zen, 1 : 1,000); anti-TRAF6 rabbit pAb (Zen, 1 : 1,000); anti-TAK1 rabbit pAb (Zen, 1 : 1,000); anti-NF- κ B-p65 mAb (Zen, 1 : 1,000); anti-MYD88 rabbit pAb (Zen, 1 : 800); anti-IRAK1 (PTG, 1 : 1,000); anti-ASC (Bioss, 1 : 1,000); anti-NLRP3 (Bioss, 1 : 1,500); and anti-caspase-1 (Bioss, 1 : 800).

Techniques: Activation Assay, Expressing